3500 Genetic Analyzer for Human Identification
The 8-capillary 3500 Genetic Analyzer sets the standard in capillary electrophoresis. The 3500-series instruments are the first genetic analyzers designed with a specific feature set and workflow for the Human Identification application.
The complete product combines the instrument with Applied Biosystems™ reagents, consumables and software, as well as industry leading support, to provide an integrated HID system solution that significantly improves ease-of-use and application efficiencies.
Key Benefits
Secure Data Management
Features include controlled access, audit trails, and e-signature support to maintain data chain of custody.
Increased Consistency
Reduced signal variation through factory standardization and optional in-lane normalization.
Real-Time Data Quality Assessment
On-instrument evaluation enables quick identification and reinjection of samples requiring further processing.
Improved Thermal Control
Enhanced oven design and door seal ensure more consistent fragment migration.
HID-Specific Workflow
Pre-configured for Applied Biosystems™ amplification kits, simplifying run setup and software navigation.
Effortless Consumable Installation
Easy-to-use, single-use consumable packaging enhances convenience and efficiency.
Consumable Tracking with RFID
Real-time monitoring of consumable usage and expiration via RFID technology built into the instrument.
Enhanced Throughput
Faster data generation using 8 or 24 capillary arrays and reduced run times.
Optimized Polymer Pump Design
Fewer bubbles and less waste result in improved data quality.
Simplified Installation and Operation
Solid-state laser eliminates the need for high-voltage wiring and external venting.
Streamlined Maintenance
HID-specific performance checks and maintenance scheduling tools reduce downtime.
Improved Thermal Control
The 3500 oven has been redesigned to provide improved temperature control for reproducible sizing precision within an injection and across injections. The newly designed oven is more compact and has an improved door seal and locking mechanism. Combined with a preheat option and a temperature-controlled detection cell heater, these features help maintain temperature consistency across the array and reduce the effects of room temperature fluctuations (Figures 1 and 2). At the cathode end, the array has been redesigned with a shorter needle length, and new array septa help to minimize exposure of the capillaries to ambient air. These thermal subsystem improvements have enabled the development and optimization of AmpFℓSTR® kit run modules with shorter run times.
Improved Signal Consistency
The 3500 Series Genetic Analyzers improve signal consistency using two methods. The first is a factory-standardized, hardware-based calibration that enhances consistency between instruments. The second is optional internal standard normalization, using the GeneScan™ 600 LIZ® Size Standard v2, which adjusts sample peak heights based on the intensity of coinjected size standard peaks. This reduces variability across instruments, injections, and capillaries. Normalization factors are calculated against a predefined target and applied to sample data. Users can enable or disable this feature, with full support in GeneMapper® ID-X Software v1.2.
Complete System Includes
- 3500 Genetic Analyzer (8-capillary configuration)
- Applied Biosystems™ reagents and consumables
- HID-optimized software
- Expert technical support and training
Primary Applications
- Human Identification (HID)
- Forensic DNA analysis
- Genetic profiling
- Laboratory quality control
Documentation and Support
- User manuals and certified protocols
- 24/7 technical support
- Training resources and installation guides